Experimental study of apoptosis and proliferation of gastric mucosal cells in chronic atrophic gastritis

• Published in: Chung-hua nei kʿo tsa chih Vol. 42; no. 2; p. 84
• Main Authors: Zhang, Ling-xia, Zhang, Li, Xu, Jun-rong, Jia, Chang-he, Zhang, Ning-xia, Cao, Guang-zhou
• Format: Journal Article
• Language: Chinese
• Published: China 01.02.2003
• Subjects: Animals; Apoptosis; Cell Division; DNA - analysis; fas Receptor - biosynthesis; Gastric Mucosa - metabolism; Gastric Mucosa - pathology; Gastritis, Atrophic - metabolism; Gastritis, Atrophic - pathology; Male; Proliferating Cell Nuclear Antigen - biosynthesis; Proto-Oncogene Proteins c-bcl-2 - biosynthesis; Rats; Rats, Sprague-Dawley
• ISSN: 0578-1426

To study the relationship between long-term high-salt hot diet and chronic atrophic gastritis (CAG). The expression of apoptosis cells, proliferation cells, bcl-2 and Fas proteins were detected in the gastric mucosa of CAG rates induced by high-salt hot water. TUNEL technique and immunohistochemical method were used in the experiment. Flow cytometry method was used to detect the DNA content of apoptosis cells. (1) The detection of apoptosis with TUNEL showed that the apoptosis cells in CAG rates induced by high-salt hot water were high. These cells could be seen in all layers of gastric mucosa, apoptosis index of model rates was higher than that of normal rates (P < 0.05). (2) The result of detecting apoptosis gene and proliferating cell nuclear antigen (PCNA) by immunohistochemical method showed that the expression of bcl-2, Fas and PCNA in the CAG rates induced by high-salt hot water was much higher than that of normal gastric mucosa (P < 0.05). The expression rates of bcl-2, Fas proteins were higher with t