Elaboration of optical immunosensors based on the surface plasmon resonance for detecting specific antibodies and antigens of Epstein-Barr virus and human adenovirus

• Published in: Mikrobiolohichnyĭ zhurnal (Kiev, Ukraine : 1993) Vol. 70; no. 6; p. 67
• Main Authors: Nesterova, N V, Nosach, L M, Zagorodnya, S D, Povnitsa, O Y, Boltovets, P M, Baranova, G V, Golovan, A V
• Format: Journal Article
• Language: English
• Published: Ukraine 01.11.2008
• Subjects: Adenoviruses, Human - immunology; Adenoviruses, Human - isolation & purification; Antibodies, Viral - immunology; Antigen-Antibody Complex - analysis; Antigen-Antibody Complex - immunology; Antigens, Viral - immunology; Biosensing Techniques - methods; Cell Line, Tumor; Herpesvirus 4, Human - immunology; Herpesvirus 4, Human - isolation & purification; Humans; Immunoenzyme Techniques; Microscopy, Electron; Sensitivity and Specificity; Spectrophotometry; Surface Plasmon Resonance
• ISSN: 1028-0987

The study of antigen-antibody interaction on the model of Epstein-Barr virus (EBV) and second type adenovirus (Ad2) based on the surface plasmon resonance (SPR) was carried out. Kinetic and concentration dependences between virus antigens and specific antisera to them at different pH were determined. Experimental samples of biosensors for the detection by SPR method of virus (EBV and Ad2) antigens using monospecific antibodies, immobilized on the surface of gold, and also for detection of specific antibodies in the blood sera of patients with EBV or adenovirus infection were elaborated