Effect of ZGDHu-1 on proliferation and apoptosis of A549 cells in vitro and antitumor activity in vivo

This study is to explore the mechanism and effect of N, N'-di-(m-methylphenyl)-3, 6-dimethyl-1, 4-dihydro-1, 2, 4, 5-tetrazine-1, 4-dicarboamide (ZGDHu-1) on proliferation and apoptosis of A549 cells in vitro and on A549 xenograft tumor in nude mice. With different concentrations of ZGDHu-1 at...

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Bibliographic Details
Published inYao hsüeh hsüeh pao Vol. 42; no. 1; p. 26
Main Authors Zhou, Yong-Lie, Hu, Wei-Xiao, Lü, Ya-Ping, Qiu, Lian-Nü, Wang, Wen-Song, Yang, Zhong-Yu, Liu, Jian-Dong, Rao, Guo-Wu
Format Journal Article
LanguageChinese
Published China 01.01.2007
Subjects
Animals
Antineoplastic Agents - pharmacology
Apoptosis - drug effects
bcl-2-Associated X Protein - biosynthesis
bcl-2-Associated X Protein - genetics
Cell Line, Tumor
Cell Proliferation - drug effects
Dose-Response Relationship, Drug
Female
Flow Cytometry
Heterocyclic Compounds, 1-Ring - pharmacology
Humans
Lung Neoplasms - metabolism
Lung Neoplasms - pathology
Lung Neoplasms - prevention & control
Mice
Mice, Inbred BALB C
Mice, Nude
Proto-Oncogene Proteins c-bcl-2 - biosynthesis
Proto-Oncogene Proteins c-bcl-2 - genetics
Random Allocation
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Tumor Suppressor Protein p53 - biosynthesis
Tumor Suppressor Protein p53 - genetics
Xenograft Model Antitumor Assays
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Summary:This study is to explore the mechanism and effect of N, N'-di-(m-methylphenyl)-3, 6-dimethyl-1, 4-dihydro-1, 2, 4, 5-tetrazine-1, 4-dicarboamide (ZGDHu-1) on proliferation and apoptosis of A549 cells in vitro and on A549 xenograft tumor in nude mice. With different concentrations of ZGDHu-1 at different times were used to treat A549 cells in vitro. The proliferation was determined by living cell count, SRB assay and Brdu-ELISA. Cell apoptosis was determined by cell morphology, DNA agarose gel electrophoresis, DNA content, Annexin V/PI and Hoechst 33258 labeling method. The nude mice model of A549 xenograft tumor was established by subcutaneous inoculation. The suppression activity of ZGDHu-1 by intraperitoneal injection on xenograft mice model was detected. The expressions of bcl-2, bax and p53 gene and protein were analyzed by RT-PCR and flow cytometry. ZGDHu-1 can inhibit A549 cell proliferation viability within a certain range of treating time and does, and a majority of A549 cells were arrested in G2-M ph
ISSN:0513-4870