Determination of propofol in human serum by improved reversed phase high-performance liquid chromatography with fluorescence detection

To develop a new high-performance liquid chromatographic (HLPC) method for determination of propofol in human serum. Human serum samples were precipitated with 20% perchloric acid and centrifuged to obtain 50 microl of the supernatant for analysis by HPLC coupled with fluorescence detection. The ana...

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Published inNan fang yi ke da xue xue bao = Journal of Southern Medical University Vol. 26; no. 10; p. 1510
Main Authors Fan, Ying-ying, Xu, Li-xian, Wen, Ai-dong, Zhang, Hui, Liu, Chun-ran, Li, Wei, Mei, Xiao-peng
Format Journal Article
LanguageChinese
Published China 01.10.2006
Subjects
Anesthetics, Intravenous - blood
Anesthetics, Intravenous - chemistry
Chromatography, High Pressure Liquid - methods
Fluorescence
Humans
Propofol - blood
Propofol - chemistry
Reproducibility of Results
Spectrometry, Fluorescence - methods
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Summary:To develop a new high-performance liquid chromatographic (HLPC) method for determination of propofol in human serum. Human serum samples were precipitated with 20% perchloric acid and centrifuged to obtain 50 microl of the supernatant for analysis by HPLC coupled with fluorescence detection. The analysis was performed with a C(18) reversed-phase column using a acetonitrile-water (90:10) phase delivered at 1.0 ml/min, with the excitation wavelength of 276 nm and emission wavelength of 310 nm. The calibration curves were linear (r=0.997 5) within the concentration range of 0.05-10 microg/ml, the limit of propofol quantification was 50 ng/ml and the intra- and inter-day precisions were between -/+15%. The method is accurate, sensitive and simple for propofol determination in clinical anesthesia.
ISSN:1673-4254