Experimental study of chronic renal tubular-interstitial injury induced by radix aristolochiae fangchi extract in rats

• Published in: Zhongguo zhongyao zazhi Vol. 30; no. 8; p. 610
• Main Authors: Du, Gui-You, Zhou, Su-Juan, Zhao, Yong, Cu, Hai-Feng, Wang, Xiu-Rong, Li, Li, Xiao, Yong-Qing, Cao, Chun-Yun, Zhang, Chun-Ying, Wu, Zi-Lun, Gao, Shuang-Rong, He, Rong, Hui, Lian-Qiang, Liu, Bao-Yan
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2005
• Subjects: Acetylglucosaminidase - urine; Animals; Aristolochia - chemistry; Aristolochia - toxicity; Aristolochic Acids - isolation & purification; Aristolochic Acids - toxicity; Blood Urea Nitrogen; Body Weight - drug effects; Dose-Response Relationship, Drug; Drugs, Chinese Herbal - administration & dosage; Drugs, Chinese Herbal - isolation & purification; Drugs, Chinese Herbal - toxicity; Female; Fibrosis - chemically induced; Kidney Tubules - pathology; Male; Mice; Plant Roots - chemistry; Plant Roots - toxicity; Plants, Medicinal - chemistry; Plants, Medicinal - toxicity; Proteinuria - chemically induced; Random Allocation; Rats; Rats, Sprague-Dawley
• ISSN: 1001-5302

To observe the acute and chronic renal toxicity induced by Radix Aristolochiae Fangchi Extract (RAFE) in different doses in rats. The conventional method of acute toxicity was used. RAFE at the dose of 25.0 mg x kg(-1) x d(-1), 120.0 mg x kg(-1) x d(-1) and 200.0 mg x kg(-1) x d(-1) and aristolochic acid (AA, 10.0 mg x kg(-1) x d(-1)) were interruptedly administrated to rats for 13 week by gastric tube, and the sample of blood, urine and kidney were collected at 4 week, 8 week and 13 week respectively. The indexes of renal function were measured and the morphology of kidney was observed. LD50 of RAFE was 36.8 g x kg(-1) (the crude drug) and the 95% confidence limit was 38.8 - 28.9 g x kg(-1). The changes of renal functions were azotemia, massive proteinuria and the increase of urinary NAGase (beta-N-acetylglucosaminidase) in the earlier period of administration with RAFE in rats. Pathological changes of renal tissue were as follows: acute renal tubular necrosis mainly in the boundary of cortex and medulla was