Study on the relationship between inheritance and immature embryo culturing capacity of maize inbreds

• Published in: Fen zi xi bao sheng wu xue bao Vol. 40; no. 2; p. 164
• Main Authors: Ma, Li, Sun, Qing Quan, Zhou, Yu Liang, Wu, Cheng Lai, Zhang, Chun Qing
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2007
• Subjects: Hybridization, Genetic; Inbreeding; Regeneration - physiology; Seeds - growth & development; Tissue Culture Techniques; Zea mays - embryology; Zea mays - genetics; Zea mays - physiology
• ISSN: 1673-520X

The immature embryo culturing capacity of maize is crucially important to maize improvement by transgenic approach. The hereditary variation regularity of maize embryo culturing capacity is not very clear yet. The research aims to study the relationship between inheritance and immature embryo culturing capacity of maize inbreds. By culturing immature embryos of 22 maize inbreds, embryonic callus induction efficiency and regenerating plant efficiency were analyzed with cluster analysis method. And the genetic relationships of 22 inbreds were analyzed using 14 SSR primers with good polymorphism. Results showed that 22 inbreds were classified into three groups. The average embryonic callus efficiency and regenerating plant efficiency of the inbreds with good culturing capacity were 85.0% and 86.7% respectively. The average embryonic callus efficiency and regenerating plant efficiency of the inbreds with middle culturing capacity were 41.3% and 53.1% respectively. The average embryonic callus efficiency and regen