Release of copper from CuT 380A co-incubated with human semen and its effect on sperm function in vitro

Release of copper and its effect on functional integrity of human sperms in vitro were assessed following co-incubation of semen with CuT 380A. After 30 min of incubation with semen, release of copper ions from CuT 380A was found to be 9.2 to 40 times higher compared to control incubations with PBS....

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Published inIndian journal of physiology and pharmacology Vol. 52; no. 3; pp. 267 - 273
Main Authors Misro, M M, Chaki, S P, Chandra, M, Maheshwari, A, Nandan, D
Format Journal Article
LanguageEnglish
Published India 01.07.2008
Subjects
Copper - analysis
Copper - pharmacology
Humans
In Vitro Techniques
Intrauterine Devices, Copper - adverse effects
Male
Semen - chemistry
Sperm Motility - drug effects
Spermatozoa - drug effects
Spermatozoa - physiology
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Summary:Release of copper and its effect on functional integrity of human sperms in vitro were assessed following co-incubation of semen with CuT 380A. After 30 min of incubation with semen, release of copper ions from CuT 380A was found to be 9.2 to 40 times higher compared to control incubations with PBS. Sperm function tests, when simultaneously performed following loss of motility in sperms (> 95%) after 120 min of copper exposure, depicted a significant (P < 0.001) reduction in sperm viability and hypo-osmotic swelling (HOS) response. However, the affected sperm populations revealed no significant alterations in other functional tests like acrosomal status or nuclear chromatin decondensation. It is therefore concluded that the high release of copper from CuT 380A drastically lowers sperm motility, viability and HOS response but only marginally affects the acrosome status or nuclear chromatin condensation in short term incubations.
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ISSN:0019-5499