Involvement of adenylyn cyclase signaling mechanisms in relaxing effect of relaxin and insulin on the rat uterus, trachea and human myometrium

Participation of adenylyl cyclase signaling mechanisms of relaxin and insulin action in their regulating influence on the process of relaxation of the rat uterine and tracheal smooth muscles and human myometrium was shown. The study was based on the discovery of novel adenylyl cyclase signaling mech...

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Published inRossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova Vol. 92; no. 7; p. 863
Main Authors Kuznetsova, L A, Fedin, A N, Chistiakova, O V, Plesneva, S A, Shpakov, A O, Pertseva, M N
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.07.2006
Subjects
Adenylyl Cyclases - metabolism
Animals
Cyclic AMP - analysis
Cyclic AMP - metabolism
Female
Humans
Insulin - pharmacology
Isoproterenol - pharmacology
Muscle Contraction - drug effects
Muscle Relaxation
Muscle, Smooth - chemistry
Muscle, Smooth - drug effects
Muscle, Smooth - enzymology
Myometrium - chemistry
Myometrium - drug effects
Myometrium - enzymology
Rats
Relaxin - pharmacology
Signal Transduction
Trachea - drug effects
Trachea - enzymology
Uterus - drug effects
Uterus - enzymology
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Summary:Participation of adenylyl cyclase signaling mechanisms of relaxin and insulin action in their regulating influence on the process of relaxation of the rat uterine and tracheal smooth muscles and human myometrium was shown. The study was based on the discovery of novel adenylyl cyclase signaling mechanisms of relaxin and insulin action in the muscle of vertebrates which involve: receptor --> Gi protein (betagamma dimer) --> phosphatidylinositol-3-kinase --> protein kinase Csigma (zeta) --> Gs protein --> adenylyl cyclase --> cAMP. In the rat uterus, trachea and human myometrium, relaxin, insulin and isoproterenol induced relaxation of KCl-contraction. The order of efficiency of the agents based upon their ability to induce the inhibiting effect on the KCl-contraction was as follows: relaxin = insulin > isoproterenol. The hormones induce activating effect on adenylyl cyclase leading to production of cAMP in the rat uterine and tracheal smooth muscles and human myometrium. It is shown that cAMP reproduces relaxing effect of the hormones under study. Thus, the involvement of novel adenylyl cyclase signaling mechanisms of relaxin and insulin action in realization of their relaxation effect on rat uterus, trachea and human myometrium was revealed for the first time.
ISSN:0869-8139