Repair of swine full-thickness cutaneous deficiency by autogenic BMSCs compounded with collagen membrane

To supply references to tissue-engineered skin clinical applications with autogenic BMSCs composited collagen membrane to repair swine full-thickness cutaneous deficiency. Twenty mL bone marrow were obtained respectively from 4 swine, autogenic BMSCs were cultured and passed to the 3rd passage. The...

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Published inZhongguo xiu fu chong jian wai ke za zhi Vol. 23; no. 3; p. 348
Main Authors He, Lijuan, Liu, Daqing, Bai, Cixian, Yan, Yingfun, Guan, Lidong, Pei, Xuetao
Format Journal Article
LanguageChinese
Published China 01.03.2009
Subjects
Animals
Bone Marrow Cells
Collagen
Female
Male
Membranes - transplantation
Mesenchymal Stem Cell Transplantation
Skin - injuries
Swine
Tissue Engineering
Transplantation, Autologous
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Summary:To supply references to tissue-engineered skin clinical applications with autogenic BMSCs composited collagen membrane to repair swine full-thickness cutaneous deficiency. Twenty mL bone marrow were obtained respectively from 4 swine, autogenic BMSCs were cultured and passed to the 3rd passage. The fresh bovine tendon treated by means of chemically cross-linked was made 5 cm diameter collagen I (Col I) membrane. The 2 x 10(7)/mL P3 swine autogenic BMSCs labeled DAPI were planted to sterile Col I membrane for 24 hours incubation, then the tissue-engineered skin was constructed. The five full-thickness skin defect of 5 cm diameter was excised to the muscle from forward to backward on the back midline two sides of swine. The tissue-engineered skin were implanted in the experimental group, while Col I membrane was implanted in control group. After 3 and 8 weeks of implantation, the two swine wound surface healing circumstance was observed and further evaluated with histology analysis and TEM. After 3 weeks of imp
ISSN:1002-1892