Effects of sensitized donor lymphocyte infusion on the chimerism and graft-versus-host disease after nonmyeloablative allogeneic stem cell transplantation

To explore whether the complete donor chimerism could be achieved and graft-versus-host disease could be alleviated by donor lymphocyte infusion which was sensitized by the skin of the recipient, female C57BL/6 mice (H-2(b), B6) as recipients received total body irradiation (TBI) of 5.5 Gy ((60)Co g...

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Published inZhongguo shi yan xue ye xue za zhi Vol. 14; no. 1; p. 102
Main Authors Chen, Bao-An, Zhang, Yan, Ding, Jia-Hua, Bi, Yan-Zhi, Zhao, Gang, Gao, Chong, Sun, Yun-Yu, Sun, Xue-Mei, Wang, Jun, Chen, Ning-Na, Cheng, Jian
Format Journal Article
LanguageChinese
Published China 01.02.2006
Subjects
Animals
CD4-CD8 Ratio
Chimerism
Female
Graft vs Host Disease - prevention & control
Graft vs Leukemia Effect
Lymphocyte Transfusion
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Stem Cell Transplantation - adverse effects
Stem Cell Transplantation - methods
Transplantation Conditioning - methods
Whole-Body Irradiation
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Summary:To explore whether the complete donor chimerism could be achieved and graft-versus-host disease could be alleviated by donor lymphocyte infusion which was sensitized by the skin of the recipient, female C57BL/6 mice (H-2(b), B6) as recipients received total body irradiation (TBI) of 5.5 Gy ((60)Co gamma-ray) on day 0 followed by allogeneic hematopoietic stem cell transplantation (allo-HSCT). The allo-grafts consisted of 2 x 10(7) peripheral hematopoietic stem cells from mobilized male BALB/c (H-2(d)) donor mice with the granulocyte colony-stimulating factor (G-CSF). Day 2 after allo-HSCT, the recipient mice were given 200 mg/kg cyclophosphamide intraperitoneally. Afterwards these recipient mice were infused 2 x 10(6) sensitized or unsensitized-donor lymphocytes at the 28 days after transplantation. The results showed that the mice receiving sensitized-donor lymphocyte infusion did not suffer from GVHD and the phenotypic character of the recipient mice (black color) converted to that of the donor mice (white c
ISSN:1009-2137