Application of histochemical staining in diagnosis of osteosarcomas

• Published in: Zhōnghuá zhŏngliú zázhì Vol. 27; no. 8; p. 489
• Main Authors: Li, Qing, Gong, Xi-qi, Ma, Fu-cheng, Zhao, Yi-ling, Zhu, Xiao-hui
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2005
• Subjects: Bone Neoplasms - pathology; Histocytochemistry; Humans; Osteosarcoma - pathology; Staining and Labeling - methods
• ISSN: 0253-3766

To study the histochemical staining in the diagnosis of osteosarcoma. To compare the effectiveness of picrosirius red, improved Ponceau trichrome and Masson trichrome staining methods on bone formation tissues in conventional osteosarcoma, paraosteal osteosarcoma, periosteal osteosarcoma, extraskeletal osteosarcoma, inflammatory myofibroblastic tumour, malignant fibrohistiocytoma, chondrosarcoma, fibrosis with ossification and calcification. With modified Ponceau trichrome staining, bone formation tissues showed a homogenous, orange-red interblended with blue in color. From osteoid to mature bone the color changed from orange-red, light blue to dark blue. Fibrotic tissue was stained blue in color with striated appearance. Cartilage was not stained. Picrosirius red method gave bone formation tissues homogenous staining. Along with bone maturation, from osteoid tissue to mineralized bones, the color showed changes from light red, yellow, orange-red, red to dark purple. The cartilage demonstrated homogenous ligh