Chemical composition and immunochemical characteristics of the lipopolysaccharide of nitrogen-fixing rhizobacterium Azospirillum brasilense CD

The chemical composition of the lipopolysaccharide of the associative diazotrophic rhizobacterium Azospirillum brasilense Cd has been studied. Among the main components of the hydrophobic part of the lipopolysaccharide, we identified 3-hydroxytetradecanoic, hexadecenoic, 3-hydroxyhexadecanoic, hexad...

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Published inMikrobiologija (Moskva. 1932) Vol. 75; no. 3; p. 383
Main Authors Konnova, O N, Burygin, G L, Fedonenko, Iu P, Matora, L Iu, Pankin, K E, Konnova, S A, Ignatov, V V
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.05.2006
Subjects
Azospirillum brasilense - chemistry
Azospirillum brasilense - classification
Carbohydrates - analysis
Chromatography, Gel
Colorimetry
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Fatty Acids - analysis
Immunodiffusion
Lipopolysaccharides - analysis
Serotyping
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Summary:The chemical composition of the lipopolysaccharide of the associative diazotrophic rhizobacterium Azospirillum brasilense Cd has been studied. Among the main components of the hydrophobic part of the lipopolysaccharide, we identified 3-hydroxytetradecanoic, hexadecenoic, 3-hydroxyhexadecanoic, hexadecanoic, octadecenoic, and nanodecanoic fatty acids; the carbohydrate part contained rhamnose, galactose, and mannose. Polyclonal antibodies against the preparation under study were raised in rabbits. Serological relations between A. brasilense Cd and other strains of Azospirillum spp. were studied using double radial immunodiffusion and enzyme-linked immunosorbent assay.
ISSN:0026-3656