Microarrays: quality control and hybridization protocol

Microarray technology is an exciting and promising tool, and is increasingly employed for studying circadian rhythms. To obtain optimal results from this technology, it is important to perform quality control experiments before engaging in genome-wide expression analysis. In this chapter, we provide...

Full description

Saved in:
Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 362; p. 225
Main Authors Uno, Ken-ichiro, Ueda, Hiroki R
Format Journal Article
LanguageEnglish
Published United States 2007
Subjects
Animals
Base Sequence
Circadian Rhythm - genetics
DNA Primers - genetics
Gene Expression Profiling
Humans
Nucleic Acid Hybridization - methods
Oligonucleotide Array Sequence Analysis - methods
Oligonucleotide Array Sequence Analysis - standards
Polymerase Chain Reaction - methods
Quality Control
Online AccessGet more information

Cover

More Information
Summary:Microarray technology is an exciting and promising tool, and is increasingly employed for studying circadian rhythms. To obtain optimal results from this technology, it is important to perform quality control experiments before engaging in genome-wide expression analysis. In this chapter, we provide an overview of quantitative polymerase chain reaction experiments using the ABI PRISM 7900HT system for quality control of samples, and microarray experiments using the Affymetrix GeneChip system for genome-wide expression analysis.
ISSN:1064-3745
DOI:10.1385/1-59745-257-2:225