Dynamic change of apoptosis of alveolar cells in ischemia-reperfusion induced pulmonary injury: an experimental study with rats

To observe the dynamic changes of alveolar apoptosis in ischemia-reperfusion (IR) induced pulmonary injury, and to evaluate the roles of these two cell death styles, apoptosis and necrosis, in the progress of lung function deterioration in pulmonary IR injury. Fifty-four Sprague-Dawley rats were mad...

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Published inZhong hua yi xue za zhi Vol. 84; no. 19; p. 1597
Main Authors Zhang, Sai, Chen, Ru-kun, Lin, Min, He, Xue-ming
Format Journal Article
LanguageChinese
Published China 02.10.2004
Subjects
Animals
Apoptosis
Female
Lung - blood supply
Lung - pathology
Male
Pulmonary Alveoli - pathology
Rats
Rats, Sprague-Dawley
Reperfusion Injury - pathology
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Summary:To observe the dynamic changes of alveolar apoptosis in ischemia-reperfusion (IR) induced pulmonary injury, and to evaluate the roles of these two cell death styles, apoptosis and necrosis, in the progress of lung function deterioration in pulmonary IR injury. Fifty-four Sprague-Dawley rats were made ischemia/reperfusion models by ischemia and reperfusion in situ in single lung. Thirty-six of the 54 rats in the experimental group were re-divided into 6 equal subgroups to undergo detection of partial pressure of oxygen (PaO2) of blood in left atrium, detection of lung tissue wet weight/dry weight ratio, histology of lung by light microscope, examination of ultrastructural changes of cells by transmission electron microscopy, and quantitative detection of apoptotic cells in the right middle lobe by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) 0 h, 0.5 h, 1 h, 2 h, 6 h, and 12 h respectively after the reperfusion (subgroups R0, R(0.5), R1, R2, R6, and R12). Another 18 rats in the
ISSN:0376-2491