Dynamic observation of vascular endothelial growth factor (VEGF)/VEGF-receptors expression in acute leukemia

• Published in: Chung-hua nei kʿo tsa chih Vol. 43; no. 11; p. 845
• Main Authors: Wang, Chen, Chen, Fang-Yuan, Zhu, Jian-Shan, Xu, Yan-Ping, Han, Jie-Ying, Ouyang, Ren-Rong
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2004
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Bone Marrow - blood supply; Bone Marrow - metabolism; Female; Humans; Immunohistochemistry; Leukemia, Myeloid, Acute - drug therapy; Leukemia, Myeloid, Acute - metabolism; Male; Middle Aged; Neovascularization, Pathologic; Precursor Cell Lymphoblastic Leukemia-Lymphoma - drug therapy; Precursor Cell Lymphoblastic Leukemia-Lymphoma - metabolism; Vascular Endothelial Growth Factor A - metabolism; Vascular Endothelial Growth Factor Receptor-1 - metabolism; Vascular Endothelial Growth Factor Receptor-2 - metabolism
• ISSN: 0578-1426

To investigate the expression of vascular endothelial growth factor (VEGF)/VEGF-receptors and its relation to bone marrow angiogenesis in acute leukemia patients before and after chemotherapy. Bone marrow biopsies from 122 cases with different stages of human acute leukemia were immunostained with anti-vascular endothelial growth factor, anti-fms-like tyrosine kinase (Flt-1) and anti- kinase-domain insert containing receptor (KDR) antibodies with envision two-step immunohistochemical staining method. The expression of VEGF and KDR protein in remission patients was 5.3 (3.3 - 9.0) and 2.0 (1.0 - 4.0) being significantly lower than newly diagnosed untreated patients 6.0 (3.3 - 12.0) and 5.3 (3.3 - 8.0) (P < 0.05, 0.01). However nonsignificant decrease was shown among non-remission patients. In relapsed patients the expression of VEGF and KDR was significantly increased as compared with that in newly diagnosed patients. Flt-1 staining levels were in the same range between the newly diagnosed untreated patients a