Study on the prevalence of the "high pathogenicity island" of Yersinia enterocolitica WA in Enterotoxigenic, Enteropathogenic and Enteroaggregative E. coli strains

• Published in: Zhōnghuá liúxíngbìng zázhì Vol. 24; no. 3; p. 213
• Main Authors: Wang, Yong, Wang, Hong, Xiang, Qian, Sun, Su-xia, Yu, Shou-yi
• Format: Journal Article
• Language: Chinese
• Published: China 01.03.2003
• Subjects: Bacterial Proteins - analysis; Bacterial Proteins - genetics; Enterotoxins - genetics; Escherichia coli - genetics; Escherichia coli - pathogenicity; Escherichia coli Infections - microbiology; Genes, Bacterial; Receptors, Cell Surface - genetics; Virulence - genetics; Yersinia enterocolitica - genetics; Yersinia enterocolitica - pathogenicity
• ISSN: 0254-6450

To detect the "high pathogenicity island" of Yersinia enterocolitica WA in E. coli and the to provide evidence for theory base of bacterial evolution process and the different structures in different E. coil. Polymerase chain reaction (PCR), nucleic acid hybridization in situ were used to detect and identify HPI. DNA sequencing was used to compare the gene homology of HPI among E. coli with Yersinia enterocolitica (Yen). The irp2 and fyua genes of Yen HPI were investigated in E. coli strains. Among them, 30 strains were isolated from 93 Enterotoxigenic E. coli (ETEC) strains and 3 strains were positive in 10 strains Enteropathogenic (EPEC). HPI was also detected in Enteroaggregative E. coli (EAggEC) strain. In most of these isolates, HPI was bordered by an asntRNA locus, as in Yersinia sp. Through sequential comparison, the gene sequence homology was higher between in EPEC and EAggEC than ETEC and Yersinia enterocolica. ETEC, EPEC and EAggEC were pathogenicity bacterias and many of them harboring HPI of Yen a