Effects of methyl jasmonat on accumulation and release of tanshinones in suspension cultures of Salvia miltiorrhiza hairy root

• Published in: Zhongguo zhongyao zazhi Vol. 32; no. 4; p. 300
• Main Authors: Wang, Xue-Yong, Cui, Guang-Hong, Huang, Lu-Qi, Qiu, De-You
• Format: Journal Article
• Language: Chinese
• Published: China 01.02.2007
• Subjects: Acetates - pharmacology; Culture Techniques; Cyclopentanes - pharmacology; Diterpenes, Abietane; Oxylipins - pharmacology; Phenanthrenes - metabolism; Plant Growth Regulators - pharmacology; Plant Roots - drug effects; Plant Roots - growth & development; Plant Roots - metabolism; Plants, Medicinal - drug effects; Plants, Medicinal - growth & development; Plants, Medicinal - metabolism; Salvia miltiorrhiza - drug effects; Salvia miltiorrhiza - growth & development; Salvia miltiorrhiza - metabolism
• ISSN: 1001-5302

To study the effects of methyl jasmonate (MJ) on the accumulation and release of tanshinones in suspension cultures of Salvia miltiorrhiza hairy roots. After 18 day's suspension culture of S. miltiorrhiza hairy roots induced by Agrobacterium rhizogenes ATCC15834, the chemical elicitor--methyl jasmonat was added into 6-7V suspension cultures and at the same time, tanshinones contents (including cryptotanshinone and tanshinone II(A)) on the day 2, 6 and 9, after dealing with MJ, was quantified by HPLC. After dealing with MJ on the day 2, 6 and 9, the concentration of cryptotanshinone reached to 0.039, 0.204, 0.571 mg x g(-1) respectively,and tanshinone II(A) reached 0.251, 0.601 and 1.563 mg x g(-1) respectively. After 9 day's treatment by MJ, the maximum increase of cryptotanshinone and tanshinone II(A) were 23.8 fold and 6.2 fold higher than that of the control respectively. MJ could stimulate the accumulation of tanshinones in S. miltiorrhiza and have released them into the culture medium.