Antisense RNA targeting survivin enhances the chemosensitivity of LOVO/Adr cells to taxotere
To investigate the ability of antisense RNA eukaryotic expression plasmid pcDNA3.0/survivin targeting survivin gene to inhibit survivin expression and enhance the sensitivity to taxotere in multidrug resistant colon carcinoma cell line LOVO/Adr. The antisense RNA eukaryotic plasmid pcDNA3.0/survivin...
Saved in:
Published in | Zhonghua wei chang wai ke za zhi Vol. 8; no. 5; p. 455 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | Chinese |
Published |
China
01.09.2005
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Abstract | To investigate the ability of antisense RNA eukaryotic expression plasmid pcDNA3.0/survivin targeting survivin gene to inhibit survivin expression and enhance the sensitivity to taxotere in multidrug resistant colon carcinoma cell line LOVO/Adr.
The antisense RNA eukaryotic plasmid pcDNA3.0/survivin was transfected into LOVO/Adr cells by lipofectamine. The expression of survivin mRNA was measured using RT-PCR. After treated with taxotere, MTT assay and flow cytometry were used to evaluate the proliferation inhibition and apoptosis of LOVO/Adr cells.
The expression of survivin mRNA in LOVO/Adr cells transfected with pcDNA3.0/survivin was down-regulated in a time- dependent manner. The inhibitory rate of taxotere (0.5 micromol/L) was (37.3 +/- 2.9)% in pcDNA3.0/survivin transfected cells, significantly higher than (21.9 +/- 2.3)% and (21.1 +/- 1.9)% in pcDNA3.0 transfected and untransfected control cells respectively (P< 0.01). The apoptosis rate of taxotere was (28.7 +/- 1.7)% in pcDNA3.0/survivin transfected |
---|---|
AbstractList | To investigate the ability of antisense RNA eukaryotic expression plasmid pcDNA3.0/survivin targeting survivin gene to inhibit survivin expression and enhance the sensitivity to taxotere in multidrug resistant colon carcinoma cell line LOVO/Adr.
The antisense RNA eukaryotic plasmid pcDNA3.0/survivin was transfected into LOVO/Adr cells by lipofectamine. The expression of survivin mRNA was measured using RT-PCR. After treated with taxotere, MTT assay and flow cytometry were used to evaluate the proliferation inhibition and apoptosis of LOVO/Adr cells.
The expression of survivin mRNA in LOVO/Adr cells transfected with pcDNA3.0/survivin was down-regulated in a time- dependent manner. The inhibitory rate of taxotere (0.5 micromol/L) was (37.3 +/- 2.9)% in pcDNA3.0/survivin transfected cells, significantly higher than (21.9 +/- 2.3)% and (21.1 +/- 1.9)% in pcDNA3.0 transfected and untransfected control cells respectively (P< 0.01). The apoptosis rate of taxotere was (28.7 +/- 1.7)% in pcDNA3.0/survivin transfected |
Author | Lian, Yan-jun Chen, Dao-da Huang, Tao |
Author_xml | – sequence: 1 givenname: Yan-jun surname: Lian fullname: Lian, Yan-jun email: lianalian@163.com organization: Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. lianalian@163.com – sequence: 2 givenname: Dao-da surname: Chen fullname: Chen, Dao-da – sequence: 3 givenname: Tao surname: Huang fullname: Huang, Tao |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/16224667$$D View this record in MEDLINE/PubMed |
BookMark | eNo1j8tKxDAYhbMYcS76CpIXKObWJF2WwRsUC6KuhCFN_kwrbTo0mcF5eyvq6izOx8c5a7QIY4AFWlGpaEaYEku0jvGTEEGLQl-iJZWMCSnVCn2UIXURQgT88lziZKY9pC7scTxOp-7UBQyhNcFCxKkFbFsYxh-8S3OZznj0uKrf69vSTdhC38_YOFu-xgQTXKELb_oI13-5QW_3d6_bx6yqH562ZZUdKBMp00WeN96ZQghNBJPKcOuMdWDmlVZyJQjjOdeeWM4K4j13mnpJnCaN4kaxDbr59R6OzQBud5i6wUzn3f9N9g236E_x |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | no_fulltext_linktorsrc |
ExternalDocumentID | 16224667 |
Genre | English Abstract Journal Article |
GroupedDBID | --- -05 2B. 92F 92I ACGFS ALMA_UNASSIGNED_HOLDINGS CCEZO CGR CIEJG CUY CVF CW9 ECM EIF F5P NPM TCJ TGQ U1G U5O |
ID | FETCH-LOGICAL-p124t-8955bfda944804267a3cdacdea224c6374023538f0c3290ff3d81f60d80b73a72 |
ISSN | 1671-0274 |
IngestDate | Tue Oct 15 23:29:00 EDT 2024 |
IsPeerReviewed | false |
IsScholarly | true |
Issue | 5 |
Language | Chinese |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-p124t-8955bfda944804267a3cdacdea224c6374023538f0c3290ff3d81f60d80b73a72 |
PMID | 16224667 |
ParticipantIDs | pubmed_primary_16224667 |
PublicationCentury | 2000 |
PublicationDate | 2005-Sep |
PublicationDateYYYYMMDD | 2005-09-01 |
PublicationDate_xml | – month: 09 year: 2005 text: 2005-Sep |
PublicationDecade | 2000 |
PublicationPlace | China |
PublicationPlace_xml | – name: China |
PublicationTitle | Zhonghua wei chang wai ke za zhi |
PublicationTitleAlternate | Zhonghua Wei Chang Wai Ke Za Zhi |
PublicationYear | 2005 |
SSID | ssj0041998 ssib001103531 ssib051368319 |
Score | 1.7002398 |
Snippet | To investigate the ability of antisense RNA eukaryotic expression plasmid pcDNA3.0/survivin targeting survivin gene to inhibit survivin expression and enhance... |
SourceID | pubmed |
SourceType | Index Database |
StartPage | 455 |
SubjectTerms | Apoptosis - drug effects Cell Line, Tumor Colonic Neoplasms - drug therapy Drug Resistance, Neoplasm Drug Screening Assays, Antitumor Humans Microtubule-Associated Proteins - genetics Microtubule-Associated Proteins - pharmacology RNA, Antisense - genetics RNA, Messenger - genetics Taxoids - pharmacology Taxoids - therapeutic use Transfection |
Title | Antisense RNA targeting survivin enhances the chemosensitivity of LOVO/Adr cells to taxotere |
URI | https://www.ncbi.nlm.nih.gov/pubmed/16224667 |
Volume | 8 |
hasFullText | |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnZ3fi9QwEMfD3vniiyj-_kUefCvRtml-9EmWU1kE90D25BThSJvEVrl28dq7Y__6mzRtt7eoqC9laZZC88nOzkxmvkHoBU-YFZQqYrM4IYnWMUmNEERZK3TIpDVdwu3Dki-OkvfH7Hg2ez2pWmqb7GW--WVfyf9QhXvA1XXJ_gPZ8aFwAz4DX7gCYbj-FeN55Y5Srs5M8HE5D3xRd5cfaMEAnDsxkKpwVM869xLwnNbu62V_YoTbHjj8dOgkFfTPwKXwO7mHRl3WMNvXaoS-FHX1rWhVcGFK3ywcXKgy-GGCjQo2RTnW9ZQ-ofpZVeR7Oy68g74H5I2qyTYFsGj7ZPVK1deyD2wsrxoMJheR2wdOphZVThYOm1jHxCvyTsisTzs0EXe6dv5cjj-P7ohjD0N7aE9IZ-CWk2QNeDaUbTc5WUS5pE69zv9RJ6690MXjwys4Adn-eTtBRudsrG6jW32UgOce-R002xR30dcRNwbceMSNB9x4wI0BN97FjWuLHe5XABt3sHFT4wH2PXT07u3qYEH6wzHIGlyyhsiUscxqlUJ87eJgoWiuVa6NgjfIORWJUzKi0oY5jdPQWqplZHmoZZgJqkR8H-1XdWUeIiy0UkLwNFEGgkttMhnlOrM8l7G2VLBH6IGfjJO1V0A5Gabp8W9HnqCb2-XyFN2w8JMzz8B_a7LnHaUrflpGYA |
link.rule.ids | 786 |
linkProvider | National Library of Medicine |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Antisense+RNA+targeting+survivin+enhances+the+chemosensitivity+of+LOVO%2FAdr+cells+to+taxotere&rft.jtitle=Zhonghua+wei+chang+wai+ke+za+zhi&rft.au=Lian%2C+Yan-jun&rft.au=Chen%2C+Dao-da&rft.au=Huang%2C+Tao&rft.date=2005-09-01&rft.issn=1671-0274&rft.volume=8&rft.issue=5&rft.spage=455&rft_id=info%3Apmid%2F16224667&rft_id=info%3Apmid%2F16224667&rft.externalDocID=16224667 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1671-0274&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1671-0274&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1671-0274&client=summon |