Antisense RNA targeting survivin enhances the chemosensitivity of LOVO/Adr cells to taxotere

• Published in: Zhonghua wei chang wai ke za zhi Vol. 8; no. 5; p. 455
• Main Authors: Lian, Yan-jun, Chen, Dao-da, Huang, Tao
• Format: Journal Article
• Language: Chinese
• Published: China 01.09.2005
• Subjects: Apoptosis - drug effects; Cell Line, Tumor; Colonic Neoplasms - drug therapy; Drug Resistance, Neoplasm; Drug Screening Assays, Antitumor; Humans; Microtubule-Associated Proteins - genetics; Microtubule-Associated Proteins - pharmacology; RNA, Antisense - genetics; RNA, Messenger - genetics; Taxoids - pharmacology; Taxoids - therapeutic use; Transfection
• ISSN: 1671-0274

To investigate the ability of antisense RNA eukaryotic expression plasmid pcDNA3.0/survivin targeting survivin gene to inhibit survivin expression and enhance the sensitivity to taxotere in multidrug resistant colon carcinoma cell line LOVO/Adr. The antisense RNA eukaryotic plasmid pcDNA3.0/survivin was transfected into LOVO/Adr cells by lipofectamine. The expression of survivin mRNA was measured using RT-PCR. After treated with taxotere, MTT assay and flow cytometry were used to evaluate the proliferation inhibition and apoptosis of LOVO/Adr cells. The expression of survivin mRNA in LOVO/Adr cells transfected with pcDNA3.0/survivin was down-regulated in a time- dependent manner. The inhibitory rate of taxotere (0.5 micromol/L) was (37.3 +/- 2.9)% in pcDNA3.0/survivin transfected cells, significantly higher than (21.9 +/- 2.3)% and (21.1 +/- 1.9)% in pcDNA3.0 transfected and untransfected control cells respectively (P< 0.01). The apoptosis rate of taxotere was (28.7 +/- 1.7)% in pcDNA3.0/survivin transfected