Study on negative regulatory factors in bone marrow mononuclear cells of myelodysplastic syndromes

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 12; no. 5; p. 610
• Main Authors: Huang, Hong-Ming, Xu, Rui-Rong, Ding, Run-Sheng, Lu, De-Yan, Liu, Hong
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2004
• Subjects: Adult; Aged; Bone Marrow Cells - metabolism; fas Receptor - genetics; Female; Humans; Interferon-gamma - biosynthesis; Male; Middle Aged; Myelodysplastic Syndromes - metabolism; Myelodysplastic Syndromes - pathology; RNA, Messenger - analysis; T-Lymphocyte Subsets - metabolism; Tumor Necrosis Factor-alpha - biosynthesis
• ISSN: 1009-2137

To explore the difference of negative regulatory factors among T lymphocyte subsets in bone marrow (BM) of myelodysplastic syndromes (MDS) and their relations to apoptotic gene Fas, different lymphocyte subsets in BM were categorized by monoclonal antibodies with 3 color fluorescence using flow cytometry, and the intracellular cytokines such as tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma) were determined following marrow cells culture. Then Fas mRNA of bone marrow mononuclear cells (BMMNC) were examined by RT-PCR. The results showed that TNF-alpha, IFN-gamma levels in BM of MDS both increased, the former produced by cells CD4+CD45RO+, CD8+CD45RO+, the latter by cells CD4+CD45RO+, CD8+CD45RO+, CD8+CD45RA+, in which the cells CD8+CD45RO+ were dominant. Fas mRNA expression had relationship with IFN-gamma produced by T cells but not with TNF-alpha. It is concluded that in hematopoietic microenvironment of MDS, not only the T lymphocyte subsets are in disorder, but also negative regulatory