Evaluation of crude and purified Toxocara canis antigens in serodiagnosis of human toxocariasis

Three serological tests: Immunodiffusion (ID), Counterimmunoelectrophoresis (CIEP) and Enzyme-linked immunosorbent assay (ELISA) were used to study the role of crude adult worm antigen (CAWA) of Toxocara canis and each of its purified fractions in the serodiagnosis of human toxocariasis. Sensitiviti...

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Bibliographic Details
Published inJournal of the Egyptian Public Health Association Vol. 71; no. 3-4; p. 321
Main Authors Abdel Aal, T M, Youssef, F G, el-Hady, H M, Sabry, N M, Habib, K S, Saber, J M
Format Journal Article
LanguageEnglish
Published England 1996
Subjects
Animals
Antigens, Helminth - chemistry
Antigens, Helminth - immunology
Antigens, Helminth - isolation & purification
Case-Control Studies
Chi-Square Distribution
Chromatography, Agarose
Counterimmunoelectrophoresis - methods
Counterimmunoelectrophoresis - standards
Cross Reactions
Egypt - epidemiology
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - standards
Humans
Immunodiffusion - methods
Immunodiffusion - standards
Sensitivity and Specificity
Serologic Tests - methods
Severity of Illness Index
Toxocara canis - immunology
Toxocariasis - blood
Toxocariasis - diagnosis
Toxocariasis - epidemiology
Toxocariasis - immunology
Egypt
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Summary:Three serological tests: Immunodiffusion (ID), Counterimmunoelectrophoresis (CIEP) and Enzyme-linked immunosorbent assay (ELISA) were used to study the role of crude adult worm antigen (CAWA) of Toxocara canis and each of its purified fractions in the serodiagnosis of human toxocariasis. Sensitivities of the three tests were lower in the ocular than in the visceral group, using different antigens. Purified fraction 1 showed more sensitive and specific reactions in the three tests, compared to CAWA or purified fraction 2 (P-F2) antigen. The other purified fractions (P-F3, P-F4 and P-F5) gave no positive reactions in any of the three tests. Using P-F1 antigen, ELISA was the most sensitive technique for diagnosis of both visceral and ocular toxocariasis followed by CIEP and then ID and the difference was statistically significant. However, CIEP was the most specific test followed by ELISA and lastly ID test. The ELISA test using Excretory-Secretory (E-S) larval antigen of Toxocara canis was less sensitive than the ELISA test using P-F1, although it was 100% specific. Thus, ELISA test using P-F1 is the test of choice for diagnosis of human toxocariasis, but when the specificity of a reaction is in doubt, CIEP test using the same antigen can be of value.
ISSN:0013-2446