Conditional targeting: inducible deletion by Cre recombinase

Tissue specific gene knockouts using Cre recombinase can have broad applicability in murine disease models of cardiovascular disease. The Cre system has been shown to have broad experimental versatility for both temporal and spatial control of gene deletion. By and large this is achieved by first ge...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 366; p. 309
Main Authors O'Neal, Kelly R, Agah, Ramtin
Format Journal Article
LanguageEnglish
Published United States 2007
Subjects
Actins - genetics
Animals
Crosses, Genetic
Gene Deletion
Gene Transfer Techniques
Humans
Integrases - physiology
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Models, Genetic
Myocardium - metabolism
Plasmids
Promoter Regions, Genetic
Recombination, Genetic
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Summary:Tissue specific gene knockouts using Cre recombinase can have broad applicability in murine disease models of cardiovascular disease. The Cre system has been shown to have broad experimental versatility for both temporal and spatial control of gene deletion. By and large this is achieved by first generating mice with an inducible tissue specific promoter for expression of Cre. These mice can then be crossed with a second line of mice where the gene of interest in 'knocked in' flanked by Cre recognition sequences Lox-P sites. The double transgenic lines are then induced, through administration of an exogenous agent, to allow tissue specific, i.e. cardiac, knockout of the gene of interest at the desired time. An experimental protocol delineating this technique is described in the chapter.
ISSN:1064-3745
DOI:10.1385/1-59745-030-8:309