Effects of gravity on proliferation and differentiation of adipose tissue-derived stem cells

Recent studies have demonstrated that culturing stem cells under altered gravity conditions modulates their proliferation and differentiation. In the current study we focused on osteogenesis. In an attempt to induce high proliferation rates and low differentiation of adipose tissue-derived stem cell...

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Bibliographic Details
Published inJournal of gravitational physiology Vol. 14; no. 1; p. P127
Main Authors Versari, S, Villa, A, Helder, M N, Doulabi, B Zandieh, van Loon, J, Bradamante, S
Format Journal Article
LanguageEnglish
Published United States 01.07.2007
Subjects
Adaptation, Physiological
Adipose Tissue - cytology
Adipose Tissue - enzymology
Adipose Tissue - metabolism
Alkaline Phosphatase - metabolism
Animals
Cell Differentiation
Cell Line
Cell Proliferation
Centrifugation
Core Binding Factor Alpha 1 Subunit - metabolism
Goats
Humans
Hypergravity
Mesenchymal Stromal Cells - enzymology
Mesenchymal Stromal Cells - metabolism
Mesenchymal Stromal Cells - physiology
Osteogenesis
Rotation
Time Factors
Weightlessness Simulation
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Summary:Recent studies have demonstrated that culturing stem cells under altered gravity conditions modulates their proliferation and differentiation. In the current study we focused on osteogenesis. In an attempt to induce high proliferation rates and low differentiation of adipose tissue-derived stem cells (AT-MSCs), we exposed them to simulated microgravity (sim-microg) and hypergravity. We used the random positioning machine (RPM) to simulate microg and the medium sized centrifuge for acceleration research (MidiCAR) for hypergravity. AT-MSCs from different origin (human and goat) seeded in OptiCells were housed in the RPM and MidiCAR and compared with suitable controls cultured under static conditions (1 g). The experiments lasted 7 or 14 days. We report data on AT-MSCs proliferation as DNA content, and on the expression of specific osteogenic markers (cbfa-1, alkaline phosphatase activity and Van Kossa staining).
ISSN:1077-9248