Biocompatibility of combined deproteinized bone coated with hepatocyte growth factor as scaffold for osteoblasts in vitro in fetal rabbits

To determine the cellular compatibility of combined deproteinized bone(DPB) coated with hepatocyte growth factor (HGF), and to observe the adherent effect of osteoblasts in response to HGF. Osteoblasts were isolated from fetal rabbits. Osteoblasts were cultured with DPB coated with HGF and deprotein...

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Published inZhong nan da xue xue bao. Journal of Central South University. Yi xue ban Vol. 33; no. 4; p. 359
Main Authors Dang, Hong-sheng, Zhao, Meng, Yan, Yong-xiang, Liu, Jia-guo
Format Journal Article
LanguageChinese
Published China 01.04.2008
Subjects
Animals
Biocompatible Materials - pharmacology
Bone and Bones - cytology
Bone Substitutes - metabolism
Cell Proliferation
Cells, Cultured
Female
Fetus
Hepatocyte Growth Factor - pharmacology
Osteoblasts - cytology
Osteogenesis
Pregnancy
Rabbits
Tissue Engineering - methods
Tissue Scaffolds
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Summary:To determine the cellular compatibility of combined deproteinized bone(DPB) coated with hepatocyte growth factor (HGF), and to observe the adherent effect of osteoblasts in response to HGF. Osteoblasts were isolated from fetal rabbits. Osteoblasts were cultured with DPB coated with HGF and deproteinized bone as experimental group and contral group, respectively. The proliferation and alkalinephosphatase activity were tested. Their growth was examined by inverted phase contrast microscope and scanning electronmicroscope. The osteoblasts were attached to the outside and inside surfaces and grew well. HGF/DPB could stimulate the alkalinephosphatase activity of the osteoblasts and improve the proliferation of the osteoblasts. HGF/DPB has good biocompatibility and bone induction. HGF could improve the adherent effect of DPB on osteoblasts, and it could be used as scaffold material for the bone tissue engineering.
ISSN:1672-7347