Research of porcine endogenous retrovirus in Shaziling pigs

To provide basic parameters of evaluating the biological safety of xenotransplantation from pig to human, ear tissues from 31 individuals were randomly collected from a Shazi Ling pig population. PCR and RT-PCR were performed to detect porcine endogenous retrovirus (PERV) proviral DNA and mRNA respe...

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Bibliographic Details
Published inYíchuán Vol. 28; no. 7; p. 799
Main Authors Xing, Xiao-Wei, Xue, Li-Qun, Huang, Sheng-Qiang, Wang, Wei
Format Journal Article
LanguageChinese
Published China 01.07.2006
Subjects
Animals
China
Endogenous Retroviruses - genetics
Endogenous Retroviruses - isolation & purification
Sus scrofa - virology
Viral Proteins - genetics
China
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Summary:To provide basic parameters of evaluating the biological safety of xenotransplantation from pig to human, ear tissues from 31 individuals were randomly collected from a Shazi Ling pig population. PCR and RT-PCR were performed to detect porcine endogenous retrovirus (PERV) proviral DNA and mRNA respectively. The sensitivity of the PCR was evaluated using a positive control. To study tissue distribution, RT-PCR of pol, gag and env was performed in the kidney, heart, liver, lung and spleen of 3 individuals. Finally, env-A, env-B and env-C were amplified, sequenced and analyzed using the BLAST software in NCBI (National Center for Biotechnology Information). The results showed PERV proviral DNA and mRNA could be detected in all 31 individuals by PCR and RT-PCR, respectively. env-A, env-B and env-C were only detected in 2 individuals, while in the other 29 individuals, only env-A and env-B but not env-C was detected. The quantity of DNA in PCR amplification of PERV genes should be more than 15 ng. RT-PCR results s
ISSN:0253-9772