L-arginine pretreatment lowers cerebral metabolism for cerebral protection during deep hypothermic circulatory arrest

• Published in: Nan fang yi ke da xue xue bao = Journal of Southern Medical University Vol. 28; no. 1; p. 102
• Main Authors: Gao, Yong, Zou, Xiao-Ming, Wang, Wu-Jun
• Format: Journal Article
• Language: Chinese
• Published: China 01.01.2008
• Subjects: Animals; Arginine - pharmacology; Arginine - therapeutic use; Brain - drug effects; Brain - metabolism; Brain Ischemia - etiology; Brain Ischemia - metabolism; Brain Ischemia - prevention & control; Circulatory Arrest, Deep Hypothermia Induced - adverse effects; Circulatory Arrest, Deep Hypothermia Induced - methods; Dogs; Female; Indazoles - pharmacology; Indazoles - therapeutic use; Male
• ISSN: 1673-4254

To evaluate the effect of L-arginine pretreatment on cerebral metabolism for cerebral protection during deep hypothermic circulatory arrest (DHCA). Fifteen healthy adult canines of either sex weighing 14.7-/+2.4 kg were randomly divided into 3 groups (n=5), namely the normal saline group, L-arginine pretreatment group (pretreated with 100 mg/kg L-arginine 60 min before DHCA), and L-arginine combined with 7- nitroindazole treatment group (pretreated with 100 mg/kg L-arginine and 25 mg/kg7-Ni 60 min before DHCA). For all the canines, extracorporeal circulation was established routinely to allow nasopharyngeal temperature reduction to 18 degrees celsius;, at which point DHCA commenced followed 90 min later by reperfusion. At 30 min before DHCA and 0, 45 and 90 min after DHCA as well as at 60 min after reperfusion initiation, blood samples were collected from the jugular vein and arterial to measure the plasma lactic acid, and the cerebral cortex of the parietal lobe was sampled determine the activity of Na(+)-K(