Malignant transformation of NIH3T3 cells induced by ectopic expression of PC-1 gene

• Published in: Zhonghua bing li xue za zhi Vol. 34; no. 1; p. 42
• Main Authors: Chang, Xiao-tong, Liang, Rui-xia, Zhou, Jian-guang, Zhang, Hao, Li, Jie-zhi, Wang, Jian, Zhang, Hui, Huang, Cui-fen
• Format: Journal Article
• Language: Chinese
• Published: China 01.01.2005
• Subjects: Animals; Cell Line, Transformed; Cell Transformation, Neoplastic; Gene Expression; Genes, Neoplasm - physiology; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Proteins - biosynthesis; Neoplasm Proteins - genetics; Neoplasm Proteins - physiology; NIH 3T3 Cells; Random Allocation; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Transfection
• ISSN: 0529-5807

To establish a mouse fibroblastic cell line stably transfected with PC-1 gene, and using such cell line to investigate tumor development and progression imposed by the ectopic expression of PC-1 gene. Eukaryotic expression vector pcDNA3.1(-)/myc-his-pc-1 was transfected into mouse fibroblast cell line NIH3T3 by lipofectamine. Stable transfectants were selected by G418. The integration and expression of ectopic PC-1 gene were analyzed by PCR and RT-PCR. Cytomorphological analysis, MTT, soft agar colony formation and nude mice tumorigenesis assay were used to evaluate the effects of PC-1 gene expression on tumor development and progression. NIH 3T3 cell lines stably expressing PC-1 gene were successfully established and confirmed by PCR and RT-PCR analyses of the integration and expression of ectopic PC-1 gene. Comparing with the parental cell line and cells transfected with control vector, the PC-1 gene transfectants acquired several phenotypes of transformed cells: increasing growth rate, ability to grow and