Expression of Bcl-2 and Fas and apoptosis of T lymphocyte in the peripheral blood of patients with end-stage renal diseases

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 39; no. 3; p. 406
• Main Authors: Xie, De-Qiong, Du, Yi-Ping, Liu, Mu, Zeng, Li, Gan, Hua, Chen, Li-Qun
• Format: Journal Article
• Language: Chinese
• Published: China 01.05.2008
• Subjects: Aged; Apoptosis; Fas Ligand Protein - biosynthesis; Female; Humans; Immunohistochemistry; Kidney Failure, Chronic - blood; Kidney Failure, Chronic - pathology; Kidney Failure, Chronic - therapy; Male; Middle Aged; Proto-Oncogene Proteins c-bcl-2 - biosynthesis; Renal Dialysis; T-Lymphocytes - metabolism; T-Lymphocytes - pathology
• ISSN: 1672-173X

To investigate the expression of Bcl-2 and Fas and the apoptosis of T lymphocyte in the peripheral blood of patients with end-stage renal diseases; and to test the impact of various dialysis membranes on the apoptosis of T lymphocyte. T lymphocyte was cultured with the stimulation of Phytohemagglutinin (PHA) for 24 h. The apoptosis of T lymphocyte was measured by flow cytometry. The expression of Bcl-2 and Fas was measured with immunohistochemical approach. A total of 10 non-dialyszed (ND) patients, 45 maintenance hemodialysis patients with cellulose acetate (CA) membrane, low-flux (PS-LF) and high-fluxpolusulfone (PS-HF) membrane, and 8 healthy volunteers (HC) participated in the study. The patients with end-stage renal diseases had greater apoptosis of T lymphocyte than healthy volunteers (P < 0.01). The patients undergoing hemodialysis with CA membrane had greater apoptosis of T lymphocyte than those with PS-LF and PS-HF membranes (P < 0.05). The expression of Bcl-2 in T lymphocyte of patients with end-sta