Relationship between the increase of hepatic D-bifunctional protein activity and bile acid biosynthesis in rats

• Published in: Zhōngguó yīxué kēxuéyuàn xuébào Vol. 27; no. 3; p. 321
• Main Authors: Shi, Ru-ling, Zhao, Chao-xian, Zhu, Hai-bao, Yang, Yuan, Wang, Su-ling, Jiang, Ling-ling
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2005
• Subjects: 17-Hydroxysteroid Dehydrogenases - metabolism; Animals; Bile Acids and Salts - biosynthesis; Cholesterol 7-alpha-Hydroxylase - analysis; Enoyl-CoA Hydratase - metabolism; Liver - metabolism; Male; Multienzyme Complexes - metabolism; Peroxisomal Multifunctional Protein-2; PPAR alpha - analysis; Random Allocation; Rats; Rats, Wistar; RNA, Messenger - analysis
• ISSN: 1000-503X

To determine the physiological role of D-bifunctional protein (DBP) in bile acid biosynthesis through investigating the effect of increasing activity of DBP on bile acid biosynthesis. Twenty male Wistar rats were divided into two groups: diethylhexyl phthalate (DEHP) group (n = 10) and control group (n = 10). Serum triglyceride, total cholesterol, hepatic DBP activity, and fecal bile acids were assayed. The mRNA levels of hepatic peroxisome proliferator-activated receptor alpha (PPARalpha), DBP, and cholesterol 7alpha-hydroxylase (CYP7A1) were detected by RT-PCR. Compared with control group, serum triglyceride level was decreased significantly and PPARalphamRNA level was increased significantly in DEHP group (P < 0.01). Together with a sharp induction of DBP mRNA expression and DBP activity in DEHP group (P < 0.01), the levels of CYP7A1 mRNA and fecal bile acids were significantly increased by 1.9 times and 1.6 times respectively compared to control group (P < 0.01). There was a significantly positive correla