Types and emergence time of YMDD motif mutation in hepatitis B virus polymerase gene during lamivudine treatment

• Published in: Zhonghua gan zang bing za zhi Vol. 11; no. 5; p. 302
• Main Authors: Liang, Wei-feng, Yang, Dan-hong, Shen, Yue-hong, Xie, Yuan-jun, Zhao, Nian-feng
• Format: Journal Article
• Language: Chinese
• Published: China 01.05.2003
• Subjects: Adolescent; Adult; Aged; Amino Acid Motifs - genetics; Child; Cloning, Molecular; DNA, Viral - genetics; Drug Resistance, Viral - genetics; Female; Gene Products, pol - genetics; Hepatitis B virus - drug effects; Hepatitis B virus - enzymology; Hepatitis B virus - genetics; Hepatitis B, Chronic - drug therapy; Hepatitis B, Chronic - virology; Humans; Lamivudine - pharmacology; Lamivudine - therapeutic use; Male; Middle Aged; Mutation; RNA-Directed DNA Polymerase - genetics; Time Factors
• ISSN: 1007-3418

To study the types and emergence time of YMDD motif mutation in hepatitis B virus (HBV) polymerase gene during lamivudine treatment. The serum samples were collected from 33 patients with HBV DNA rebounding and 2 non-responders after at least one year lamivudine treatment. HBV polymerase gene was amplificated by PCR, then the products were detected by restriction fragment length polymorphism (RFLP) and by direct sequence analysis. The variants with YMDD mutation were 14 out of the 35 patients. Mutation patterns detected in these patients included four YIDD, six YVDD, three YI/VDD and one YI/MDD. The mean emergence time of YMDD variants was 11.07+/-3.65 months after the treatment, and the earliest one and the latest one occurred 5 months and 17 months after the treatment respectively. The emergence times of YIDD, YVDD, YI/VDD were (10.00 +/- 1.41) months, (11.67 +/- 4.41) months and (13.33 +/- 3.31) months respectively, which had no statistical significance (F = 0.543, P < 0.05). Three patients treated with la