Influence of maternal age on meiotic spindle and chromosome configuration of oocytes

To study the effect of maternal age on meiotic spindle and chromosome configuration of oocytes. Spindle and chromosome configuration was examined in day 1 unfertilized human oocytes after in vitro fertilization (IVF) and intracytoplasmic sperm injection(ICSI) by immunocytochemistry and visualized by...

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Published inZhonghua yi xue yi chuan xue za zhi Vol. 24; no. 1; p. 6
Main Authors Chen, Wen, Hu, Juan, Liu, Qun, Wei, Yu-lan, Zhu, Gui-jin
Format Journal Article
LanguageChinese
Published China 01.02.2007
Subjects
Adult
Age Factors
Chromosomes, Human - metabolism
Female
Fertilization in Vitro
Humans
Immunohistochemistry
Microscopy, Confocal
Oocytes - metabolism
Sperm Injections, Intracytoplasmic
Spindle Apparatus - metabolism
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Summary:To study the effect of maternal age on meiotic spindle and chromosome configuration of oocytes. Spindle and chromosome configuration was examined in day 1 unfertilized human oocytes after in vitro fertilization (IVF) and intracytoplasmic sperm injection(ICSI) by immunocytochemistry and visualized by laser confocal microscopy. Statistically significant differences were observed on normal spindle and chromosome configurations of oocytes between 25-29 maternal age group (33% and 31%, respectively), and 30-34 age group (P< 0.05) as well as 35-40 age group(0%, P<0.01). The incidence of abnormal spindle and chromosome configurations of oocytes from 30-34 and 35-40 maternal age groups was much higher than that of oocytes from 25-29 age group (P<0.01, P<0.05). Incidence of abnormal spindle and chromosome configuration of oocytes is related to maternal age. It could be an important reason of age related oocyte aneuploidy.
ISSN:1003-9406