Effects of moxibustion on cerebral cortical nitric oxide level and nitric oxide synthase activity in aging mice

• Published in: Zhēncì yánjiū Vol. 33; no. 4; p. 255
• Main Authors: Zhao, Li-hua, Wen, Jian-jun, Yang, Ke, Li, Ai-yuan
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2008
• Subjects: Aging - metabolism; Animals; Cerebral Cortex - metabolism; Female; Humans; Mice; Moxibustion; Nitric Oxide - metabolism; Nitric Oxide Synthase Type I - metabolism; Random Allocation
• ISSN: 1000-0607

To explore the anti-aging effect of moxibustion of "Zusnali" (ST 36) and "Xuanzhong" (GB 39) in D-galactose induced aging mice. Thirty-three female Kunming mice (3 months old) were randomly divided into model group (n=11), saline control group (n=10), and moxibustion group (n=11). In addition, another 10 female mice (16 months old) were used as the physio-senility control (PSC) group. Senescent mouse model was established by subcutaneous injection of D-galactose (120 mg/kg x d(-1)) for 42 days. From the 13th day on after the first injection, moxibustion (3 cones/acupoint) was applied to bilateral ST36 and GB39, once every other day for one month. At the end of the treatment, the mice were killed under anesthesia for collecting cerebral cortex and cerebellar cortex separately. NO content and NOS activity were assayed by using nitrate reductase method and chemico-chromatometry respectively after homogenate and centrifugalization. Compared with saline control group, cerebral NO contents and NOS activity in physi