Cold storage of rabbit platelet suspension by adding uridine diphosphate galactose

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 14; no. 6; p. 1244
• Main Authors: Pei, Xiao-Ping, Chen, Bao-An, Huang, Cheng-Yin, Li, Cui-Ping, Shi, Guang-Yao, Xiao, Jian-Yu, Gao, Chong, Ding, Jia-Hua, Fei, Fei, Wang, Jun, Sun, Yun-Yu, Cheng, Jian, Zhao, Gang
• Format: Journal Article
• Language: Chinese
• Published: China 01.12.2006
• Subjects: Animals; Blood Platelets; Blood Preservation - methods; Cellular Senescence - drug effects; Cryopreservation - methods; Platelet Aggregation - drug effects; Rabbits; Time Factors; Uridine Diphosphate Galactose - pharmacology
• ISSN: 1009-2137

This study was aimed to investigate the method to cold-store platelets with uridine diphosphate galactose (UDP-Gal). Rabbit heart blood was prepared for concentrated platelet suspension to which UDP-Gal was added, and then stored for ten days in 4 degrees C refrigerator. Thereafter, platelet count, mean platelet volume (MPV), platelet distributing width (PDW), platelet aggregation function, platelet activity to urge coagulation including PF3aT and APCT and apoptosis were determined. Meanwhile, survival time in vivo was tested after cold-stored rabbit platelets labeled with Cr51 were transfused into rabbits. The results showed that there was not significant difference for Plt count, MPV, PDW, PF3aT and APCT between UDP-Gal cold-stored platelet group and fresh platelet group (P > 0.05). On the contrary, platelet count decreased significantly, MPV, PDW jumped and PF3aT and APCT went down in cold control group as compared with fresh platelet group (P < 0.01). Apoptosis increased in UDP-Gal cold-stored platelet gr