Ultrastructure of diatom Synedra acus subsp. radians as revealed by transmission electron microscopy after mild silica dissolution

A diatom Synedra acus subsp. radians (Kütz.) Skabitsch. has been studied by transmission electron microscopy. Examination of ultrathin sections demonstrated that silica dissolution in ammonium fluoride pH 5 under mild conditions leaves the key ultrastructural elements intact. The ultrastructure and...

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Bibliographic Details
Published inIzvestiia Akademii nauk. Seriia biologicheskaia no. 3; p. 367
Main Authors Bedoshvili, E D, Likhoshvaĭ, E V, Grachev, M A
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.05.2007
Subjects
Diatoms - chemistry
Diatoms - ultrastructure
Microscopy, Electron, Transmission
Organelles - diagnostic imaging
Silicon Dioxide - chemistry
Ultrasonography
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Summary:A diatom Synedra acus subsp. radians (Kütz.) Skabitsch. has been studied by transmission electron microscopy. Examination of ultrathin sections demonstrated that silica dissolution in ammonium fluoride pH 5 under mild conditions leaves the key ultrastructural elements intact. The ultrastructure and arrangement of the cell organelles was studied during ontogeny. Silicalemma-surrounded silica deposition vesicles (SDVs) with maturating daughter valves and forming girdle bands have been identified. This method of SDV visualization offers considerable advantages over the standard approach without silica dissolution.
ISSN:1026-3470