Apoptosis of pulmonary alveolar macrophages in aged and adult rats: a comparative study

To investigate the role of alveolar macrophages (AM) in the initiation of multiple organ failure in the elderly (MOFE). Three-month-old (adult) and 24-month-old (aged) males SD rats were used as experimental animals. Zymosan 0.5 g/kg was used to establish animal model of MOFE, normal saline were use...

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Published inZhong hua yi xue za zhi Vol. 85; no. 4; p. 253
Main Authors Qian, Xiao-shun, Zhu, Qing-lei, Yang, Jie, Yin, Tong, Wang, Shi-wen
Format Journal Article
LanguageChinese
Published China 26.01.2005
Subjects
Aging
Animals
Apoptosis - physiology
Bronchoalveolar Lavage Fluid - cytology
Cells, Cultured
Macrophages, Alveolar - cytology
Male
Pulmonary Alveoli - cytology
Rats
Rats, Sprague-Dawley
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Summary:To investigate the role of alveolar macrophages (AM) in the initiation of multiple organ failure in the elderly (MOFE). Three-month-old (adult) and 24-month-old (aged) males SD rats were used as experimental animals. Zymosan 0.5 g/kg was used to establish animal model of MOFE, normal saline were used among the control rats. The rats were divided into 4 groups: aged model group, aged control group, adult model group, and adult control group. Twenty-four hours after the establishment of model, 6 surviving rats from each group were killed. The trachea, bronthi, and lungs were isolated and lavaged with normal saline. One minute later the bronchi-alveolar lavage fluid (BALF) was re-extracted. Cells were collected from the fluid and put into 24-well cell culture plate. The alveolar macrophages (AMs) adhered to the wall were collected, suspended again, cultured, re-collected, centrifuged, and isolated. Apoptosis of the enriched AM was measured by propidium staining and flow cytometry. Fluo-3.AM staining and flow cyt
ISSN:0376-2491