Effects of acupuncture and moxibustion on DNA excision repair-related proteins of bone marrow cell in cyclophosphamide-induced mice

• Published in: Zhōngguó zhēnjiǔ Vol. 29; no. 10; p. 821
• Main Authors: Lu, Mei, Cao, Da-ming, Li, Dao-ming, Zhao, Xi-xin, Li, Jian-wei, Li, Hui-xin, Zhang, Huan-huan, Zhang, Hui-fang
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2009
• Subjects: Acupuncture Therapy; Animals; Bone Marrow Cells - enzymology; Cyclophosphamide - pharmacology; DNA Damage - drug effects; DNA Repair; DNA Repair Enzymes - genetics; DNA Repair Enzymes - metabolism; Gene Expression - drug effects; Male; Mice; Moxibustion; Random Allocation
• ISSN: 0255-2930

To explore the molecular biological mechanism of acupuncture and moxibustion for relieving myelosuppression and increasing white blood cells. Two hundred and twenty-four clean male Kunming mice were randomly divided into a control group, a model group, an acupuncture group and a moxibustion group, 56 mice in each group. The model of myelosuppression was made with Cyclophosphamide. In the acupuncture group and the moxibustion group, acupoints "Dazhui" (GV 14), "Geshu" (BL 17), "Shenshu" (BL 23) and "Zusanli" (ST 36) were used for treatment with acupuncture and moxibustion, respectively, while, in the control group and the model group, there were no treatment carried out except catching and fixing. The changes of bone marrow cell DNA pol beta and XPD between the 2nd and 7th day were examined with immunohistochemical method. Acupuncture and moxibustion markedly up-regulated the expression of bone marrow cell DNA pol beta and XPD, and promoted the base excision repair and nucleotide excision repair, which leads t