Strategies for measurement of biotransformation enzyme gene expression

The analysis of gene expression is an integral part of any research characterizing gene function. A wide variety of techniques have been developed for this purpose, each with their own advantages and limitations. This chapter seeks to provide an overview of some of the most recent as well as convent...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 291; p. 387
Main Authors Romkes, Marjorie, Buch, Shama C
Format Journal Article
LanguageEnglish
Published United States 2005
Subjects
Animals
Biotransformation - genetics
Blotting, Northern
Enzymes - biosynthesis
Enzymes - genetics
Expressed Sequence Tags
Gene Expression
Gene Expression Profiling - methods
Humans
Oligonucleotide Array Sequence Analysis
RNA, Messenger - analysis
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Summary:The analysis of gene expression is an integral part of any research characterizing gene function. A wide variety of techniques have been developed for this purpose, each with their own advantages and limitations. This chapter seeks to provide an overview of some of the most recent as well as conventional methods to quantitate gene expression. These approaches include Northern blot analysis, ribonuclease protection assay (RPA), reverse transcription polymerase chain reaction, expressed sequence tag (EST) sequencing, differential display, cDNA arrays, and the serial analysis of gene expression (SAGE). Current applications of the information derived from gene expression studies require assays to be adaptable for the quantitative analysis of a large number of samples and end points within a short period coupled with cost effectiveness. A comparison of some of these features of each analytical approach as well as their advantages and disadvantages has also been provided.
ISSN:1064-3745