Expression of KISS-1 and GnRH in rat hypothalamus

To assay the expression of KiSS-1 and GnRH in the male rat hypothalamus at different developmental stages, and to explore the significance of KiSS-1 in sex development onset and normal reproduction regulation. Expression analyses of KiSS-1 and GnRH genes were conducted in the rat hypothalamus at dif...

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Published inZhonghua nan ke xue Vol. 13; no. 4; p. 315
Main Authors Wang, Xue-song, Zhang, Cai-tian, Ju, Xiao-bin, Zhang, Zhao-hui, Ding, Gui-peng, Chen, Zi-qing, Su, En-ben, Cui, Yu-gui
Format Journal Article
LanguageChinese
Published China 01.04.2007
Subjects
Animals
Gonadotropin-Releasing Hormone - biosynthesis
Gonadotropin-Releasing Hormone - genetics
Hypothalamus - metabolism
Kisspeptins
Male
Proteins - metabolism
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
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Summary:To assay the expression of KiSS-1 and GnRH in the male rat hypothalamus at different developmental stages, and to explore the significance of KiSS-1 in sex development onset and normal reproduction regulation. Expression analyses of KiSS-1 and GnRH genes were conducted in the rat hypothalamus at different developmental stages with RT-PCR and real time-PCR. The testosterone level was assayed by chemoluminescence technique. KiSS-1 mRNA rose gradually during sex development in the rat hypothalamus, highest at puberty and lowered a little at adulthood. KiSS-1 mRNA of the prepubertal, early pubertal, pubertal and adult rats was 1.7, 2.1, 3.5 and 2.0 times higher than that of the infantile rats respectively. The expression of GnRH and KiSS-1 correlated positively (r = 0.905, P < 0.05). But the activation of GnRH neuron was later than KiSS-1. The expression of GnRH was the highest in the puberty rats. GnRH mRNA of the prepubertal, early pubertal, pubertal and adult rats was 1.1, 1.94, 2.42 and 1.92 times higher than
ISSN:1009-3591