Research of polysaccharide from Dioscorea opposita Thunb

To isolate a homogeneous polysaccharide RP from RDP (a crude polysaccharide from Dioscorea opposita Thunb.) , and study its preliminary composition and structure. RP was obtained with water extraction, alcohol sedimentation, CTAB deprotein, cellulose column and SephadexG-100 column. The purities of...

Full description

Saved in:
Bibliographic Details
Published inZhong yao cai Vol. 29; no. 9; p. 909
Main Authors Xu, Qin, Xu, Zeng-lai, Shen, Zhen-guo, Shi, Yun-yun
Format Journal Article
LanguageChinese
Published China 01.09.2006
Subjects
Dioscoreaceae - chemistry
Galactose - analysis
Glucose - analysis
Mannose - analysis
Molecular Weight
Plant Tubers - chemistry
Plants, Medicinal - chemistry
Polysaccharides - chemistry
Polysaccharides - isolation & purification
Spectrophotometry, Infrared
Spectrophotometry, Ultraviolet
Online AccessGet more information

Cover

More Information
Summary:To isolate a homogeneous polysaccharide RP from RDP (a crude polysaccharide from Dioscorea opposita Thunb.) , and study its preliminary composition and structure. RP was obtained with water extraction, alcohol sedimentation, CTAB deprotein, cellulose column and SephadexG-100 column. The purities of RP were identified by SephadexG-200. PC analysis on its acidic hydrolysates was used to determine the sugar components. Sephadex chromatography was used to mensurate its molecular weight. IR was used to analysis RP. RP was homogeneous. IR indicated that RP had beta configurations. The compositon was identified by paper chromatography as glucose, D-mannose, D-galactose. The research could provide a theoretical foundation for further development and utilization.
ISSN:1001-4454