Oligonucleotide microarray for human immunodeficiency virus detection

To develop an oligonucleotide microarray for fast detection of human immunodeficiency virus (HIV). With complete genome sequence of HIV-1 subtype B (U26942) as the target sequence and bioinformatics software such as DNAClub, Oligo6.0, BLAST, Alignment, oligonucleotide probes of high specificity with...

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Bibliographic Details
Published inDi 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Vol. 25; no. 3; p. 293
Main Authors Wen, Ying, Ma, Wen-Li, Li, Ling, Wu, Qing-Hua, Xu, Qiu-Lin, Zhang, Hai-Yan, Zheng, Wen-Ling
Format Journal Article
LanguageChinese
Published China 01.03.2005
Subjects
Base Sequence
HIV Infections - virology
HIV-1 - isolation & purification
Humans
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis
Sensitivity and Specificity
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Summary:To develop an oligonucleotide microarray for fast detection of human immunodeficiency virus (HIV). With complete genome sequence of HIV-1 subtype B (U26942) as the target sequence and bioinformatics software such as DNAClub, Oligo6.0, BLAST, Alignment, oligonucleotide probes of high specificity with identical length and similar melting temperature (T(m)) were designed and synthesized. Oligonucleotide microarray was prepared using Cartesian Microarrayer. Using the plasmids of HIV-1 subtype B(U26942), C(U46016), F(AF075703), G(AF061640) and restriction display technique, Cy3-labeled HIV DNA fragments were amplified and the hybridization results were scanned and analyzed with Array-Pro. Twenty-two optimized oligonucleotide microarray probes were obtained and used to prepare the oligonucleotide microarray for further screening studies. The microarray prepared significantly enhanced the sensitivity, reliability and speed of DNA assay, and possesses the potential for application in clinical setting.
ISSN:1000-2588