Effects of perfluorooctane sulfonate on Glu, PKC and PKA activities in mouse brain

• Published in: Zhōnghuá yùfáng-yīxué zázhì Vol. 41; no. 6; p. 466
• Main Authors: Wang, Ke, Jin, Yi-He, Yu, Qi-Lin, Liu, Li, Zhao, Cui-Xia
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2007
• Subjects: Alkanesulfonic Acids - toxicity; Animals; Brain - drug effects; Brain - metabolism; Brain Chemistry; Cyclic AMP-Dependent Protein Kinases - metabolism; Fluorocarbons - toxicity; Glutamic Acid - metabolism; Male; Mice; Mice, Inbred Strains; Protein Kinase C - metabolism
• ISSN: 0253-9624

To study the effects of perfluorooctane sulfonate (PFOS) on contents of glutamate and activity of protein kinase C (PKC) and A (PKA) and ultrastructure injury in the brain of male mice and to explore the mechanism of neurotoxicity and patho-alteration resulted from PFOS. 44 male mice were randomly divided into four groups, who were respectively orally given 0, 5, 10, 20 mg/kg PFOS for 10 days. The Glu consents in the brain of the mice was measured with spectrophotometer and protein kinases activity were measured with non-radioactive assay of protein kinase and the changes of cerebral cortex ultrastructure were observed. Contents of Glu in 10 and 20 mg/kg groups were (1.57 +/- 0.11) and (1.62 +/- 0.16) mmol/g prot respectively,which was significantly increased compared with the corresponding controlled group [(1.45 +/- 0.13) mmol/g prot] (F = 39.59, P < 0.05). PKC activity in 5, 10 and 20 mg/kg BW groups were (29.05 +/- 2.89), (33.65 +/- 3.82) and (34.20 +/- 3.16) pmol x min(-1) x (mg prot)-1 respectively, whi