Study on intrauterine infection of hepatitis B virus in pregnant women with hepatitis B surface antigen and hepatitis B e antigen negative

• Published in: Chung-hua fu chʿan kʿo tsa chih Vol. 40; no. 2; p. 99
• Main Authors: Gui, Qiao-di, Yue, Ya-fei, Li, Shu-hong, Zhang, Fen
• Format: Journal Article
• Language: Chinese
• Published: China 01.02.2005
• Subjects: Adult; DNA, Viral - analysis; DNA, Viral - blood; Female; Hepatitis B - blood; Hepatitis B - transmission; Hepatitis B e Antigens - blood; Hepatitis B Surface Antigens - blood; Hepatitis B virus - immunology; Humans; Infant, Newborn; Infectious Disease Transmission, Vertical - prevention & control; Leukocytes, Mononuclear - virology; Polymerase Chain Reaction - methods; Pregnancy; Pregnancy Complications, Infectious - prevention & control; Pregnancy Complications, Infectious - virology
• ISSN: 0529-567X

To explore the significance of intrauterine infection of hepatitis B virus in pregnant women with hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) negative by nested polymerase chain reaction (PCR). Twenty-four pregnant women with HBsAg and HBeAg negative but other HBV markers positive together with their infants were included as study group. Sixteen pregnant women with HBV marker negative and their infants were in the control group. HBV DNA in sera and peripheral blood mononuclear cells (PBMCs) of two groups was detected by nested PCR respectively. (1) In the study group, the positive rates of HBV DNA of pregnant women were 33% (8/24) in the sera and 42% (10/24) in PBMCs. Three women were detected HBV DNA in both serum and PBMCs. The rate of HBV infection was 63% (15/24); (2) also in the study group, the positive rates of HBV DNA of the infants were 13% (3/24) in the sera and 25% (6/24) in PBMCs. One newborn was detected HBV DNA in both serum and PBMCs, the rate of intrauterine infection