Effects of amiodarone on funny current I(f) channel gene expression in neonatal rat ventricular myocytes

• Published in: Zhōnghuá xīnxuèguănbìng zázhì Vol. 35; no. 5; p. 466
• Main Authors: Li, Hong-Xia, Yang, Xiang-Jun, Han, Lian-Hua, Zhou, Ya-Feng, Zhao, Xin, Jiang, Bin, Dong, Ning-Zheng, Song, Jian-Ping, Liu, Zhi-Hua, Jiang, Wen-Ping
• Format: Journal Article
• Language: Chinese
• Published: China 01.05.2007
• Subjects: Amiodarone - pharmacology; Animals; Animals, Newborn; Cells, Cultured; Cyclic Nucleotide-Gated Cation Channels - genetics; Cyclic Nucleotide-Gated Cation Channels - metabolism; Female; Gene Expression; Heart Ventricles - metabolism; Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; Male; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - metabolism; Patch-Clamp Techniques; Potassium Channels - genetics; Potassium Channels - metabolism; Rats; Rats, Sprague-Dawley
• ISSN: 0253-3758

To analysis the effect of amiodarone on funny current (I(f)) and hyperpolarization-activated cation channel (HCN) gene expressions of the neonatal rat ventricular myocytes. Ventricular myocytes of 1 - 3 days-old rats were isolated and cultured. The cardiomyocytes were treated by amiodarone (0.01, 0.1, 1, 10, 100 micromol/L) for 3 hours or amiodaron (10 micromol/L) for 0, 0.5, 1, 3, 6 hours. The I(f) and HCN 1 - 4 gene expressions were measured through the whole-cell configuration of the patch-clamp technique and real-time quantitative polymerase chain reaction (real-time PCR) using SYBR Green PCR kit. (1) HCN1, HCN2, HCN3 and HCN4 represented (0.23 +/- 0.01)%, (83.58 +/- 0.04)%, (0.79 +/- 0.01)% and (15.44 +/- 0.01)% of total HCN mRNA, respectively. (2) Amiodaron resulted in a dose-dependent I(f) [(3.1 +/- 0.9)%, (9.7 +/- 2.4)%, (36.7 +/- 5.8)%, (80.3 +/- 1.8)% and (85.9 +/- 3.1)%, respectively at -145 mV, IC(50) (1.32 +/- 0.28) micromol/L], HCN2 [(2.1 +/- 0.8)%, (8.9 +/- 3.6)%, (30.1 +/- 4.2)%, (78.3 +/- 3.6