The effect of alendronate on arterial calcification in rat model

• Published in: Chung-hua nei kʿo tsa chih Vol. 45; no. 6; p. 489
• Main Authors: Li, Huan, Jia, Guo-liang, Wang, Hai-chang, Zhang, Rong-qing, Tao, Hui-ren, Lü, Rong, Hu, Tao, Wang, Jun
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2006
• Subjects: Alendronate - therapeutic use; Animals; Aortic Diseases - drug therapy; Aortic Diseases - pathology; Bone Density Conservation Agents - metabolism; Bone Density Conservation Agents - therapeutic use; Calcinosis - drug therapy; Calcinosis - pathology; Cell Proliferation - drug effects; Male; Random Allocation; Rats; Rats, Sprague-Dawley
• ISSN: 0578-1426

To study the effect of alendronate on artery calcification in rats. (1) 4-week SD male rats were randomly divided into 3 groups: alendronate group (AL, n = 6), calcification group (CA, n = 6) and normal group (N, n = 6). In AL and CA group, artery calcification of rat was established by subcutaneous injection of vitamin D3 (300,000 U x kg(-1) x d(-1) for 3 days) and Warfarin (15 mg x 100 g(-1) x 12 h(-1) for 4 days); In AL group, at 4 days before establishment of artery calcification, alendronate (1 mg x kg(-1) x 24 h(-1)) was administered with subcutaneous injection and continued to be given to the end of the study. Abdominal aortae were collected for paraffin section and stained with von Kossa staining to observe the area of calcification. (2) Rat aortic vascular smooth muscle cells (VSMC) were cultured in vitro with tissue explant. All cells were divided into 5 groups: normal group, calcification group (control group), and alendronate 10(-9), 10(-7) and 10(-5) mol/L group. Before inducing calcification, al