Effects of rapamycin on Rho-kinase and p27 mRNA expressions in a porcine coronary intimal proliferation model induced by interleukin-1beta

• Published in: Zhōnghuá xīnxuèguănbìng zázhì Vol. 34; no. 5; p. 445
• Main Authors: Miao, Zhi-lin, Zeng, Ding-yin, Sun, Xi-zhuo, Zhou, Xu-chen, Cheng, Ying, Guan, Qi-gang, Zhang, Li, He, Xue-zhi, Han, Feng-tong
• Format: Journal Article
• Language: Chinese
• Published: China 01.05.2006
• Subjects: Animals; Coronary Angiography; Coronary Vessels - drug effects; Coronary Vessels - metabolism; Coronary Vessels - pathology; Disease Models, Animal; Interleukin-1beta - pharmacology; Male; rho-Associated Kinases - metabolism; RNA, Messenger - metabolism; Sirolimus - pharmacology; Swine; Tunica Intima - drug effects; Tunica Intima - metabolism; Tunica Intima - pathology
• ISSN: 0253-3758

To observe the effects of rapamycin on the expressions of Rho-kinase and p27 mRNA during vascular intimal proliferation in a porcine model of coronary stenosis induced by interleukin-1beta (IL-1beta). The proximal segments of LAD and LCX were wrapped with cotton mesh that had absorbed sepharose bead solution with or without IL-1beta. Selective coronary angiography was performed two weeks later and the animals were killed for collecting the samples for histopathology and RT-PCR analyzing of Rho-kinase and p27 mRNA. The expressions of Rho-kinase and p27 mRNA could be visualized in normal coronary wall. The expression of Rho-kinase mRNA was significantly enhanced and the expression of p27 mRNA was significantly decreased during the process of intimal proliferation induced by IL-1beta. Rapamycin significantly inhibited the intimal proliferation, reduced the infiltration of inflammatory cells, reduced the expression of Rho-kinase mRNA and increased the expression of p27 mRNA. The expression of Rho-kinase mRNA is u