Pretreatment of donor dendritic cells with NBD-peptide prolongs mouse cardiac allograft survival

• Published in: Nan fang yi ke da xue xue bao = Journal of Southern Medical University Vol. 28; no. 9; p. 1562
• Main Authors: Su, Gang, Zhao, Wen-zeng, Chen, Jia-jun, Xu, Jing, Qiao, Chen-hui, Liu, Chao, Sun, Zong-quan
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2008
• Subjects: Animals; Dendritic Cells - cytology; Dendritic Cells - immunology; Dendritic Cells - transplantation; Graft Rejection - immunology; Graft Survival - immunology; Heart Transplantation - immunology; Heart Transplantation - methods; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Peptides - immunology; Transplantation, Homologous
• ISSN: 1673-4254

To observe the effects of NBD-peptide pretreatment of the donor dendritic cells in immune tolerance induction in mouse allograft recipients and investigate the mechanisms. BALB/c mouse DCs pretreated with NBD-peptide (NBD-Peptide-DC) were injected into the recipient C57BL/6 mice 7 days before transplantation. Cervical heterotopic heart transplantation model was established using the cuff technique and the cardiac allograft survival time was observed. Pathological analysis were performed to examine the graft injection and the responsiveness of the recipient spleen T cell to the donor alloantigen was determined by mixed lymphocyte reaction (MLR). The serum levels of cytokines were determined using ELISA. The cardiac allograft survival time in the NBD-Peptide-DC-treated group (21.83-/+3.54 days) was significantly longer than that in the Day9-DC group (13.33-/+2.58 days) and PBS-treated group (6.66-/+1.21 days) (P<0.01), with also significantly lower pathological grade for graft rejection (P<0.01). The donor-deri