Expression of microRNA-21 in invasive ductal carcinoma of the breast and its association with phosphatase and tensin homolog deleted from chromosome expression and clinicopathologic features

• Published in: Zhong hua yi xue za zhi Vol. 88; no. 40; p. 2833
• Main Authors: Huang, Guan-li, Zhang, Xiao-hua, Guo, Gui-long, Huang, Ka-te, Yang, Kai-yan, Hu, Xiao-qu
• Format: Journal Article
• Language: Chinese
• Published: China 04.11.2008
• Subjects: Adult; Aged; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Carcinoma, Ductal, Breast - genetics; Carcinoma, Ductal, Breast - metabolism; Carcinoma, Ductal, Breast - pathology; Female; Gene Expression; Humans; MicroRNAs - biosynthesis; MicroRNAs - genetics; Middle Aged; Neoplasm Invasiveness; PTEN Phosphohydrolase - genetics
• ISSN: 0376-2491

To investigate the expression of microRNA-21 (mir-21) in invasive ductal carcinoma (IDC) of the breast and its association with phosphatase and tensin homologue deleted from chromosome (PTEN)-10 protein expression and the clinicopathologic features of IDC. Specimens of IDC and normal tissues more than 5 cm away from the tumor tissues were collected from 40 IDC patients, all female, aged 53 (35-77). Stem-loop real-time RT-PCR was used to examine the mir-21 expression. Immunohistochemistry was used to examine the PTEN protein expression in the tumor tissue. The association of mir-21 expression with the PTEN expression and the clinicopathologic features of the breast IDC were analyzed. Compared with the nontumor control samples, the median (M) of relative expression of mir-21 (2(-DeltadeltaCt)) was 5.770 (25th-75th percentile, 3.605-7.255) in the tumor samples, significantly higher than that of the nontumor control samples (set at 1.000, P<0.001). Reduced PTEN protein expression was seen in 45% (18/22) of all ca