Establishment of human embryonic stem cell line NJGLLhES1

To report the derivation and characterization of a new human embryonic stem cell (hESC) line NJGLLhES1. From the inner cell mass of frozen-thawed human embryos and with ICR mouse embryonic fibroblasts as the feeder layer, we established a new human embryonic stem cell line, which was named NJGLLhES1...

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Published inZhonghua nan ke xue Vol. 14; no. 12; p. 1083
Main Authors Sun, Hai-Xiang, Zhao, Xia, Hu, Ya-Li, Zhang, Ning-Yuan, Wang, Bin, Chen, Hua, Wang, Jun-Xia, Xu, Zhi-Peng, Chen, Lin-Jun
Format Journal Article
LanguageChinese
Published China 01.12.2008
Subjects
Animals
Cell Culture Techniques
Cell Differentiation
Cell Line
Embryo, Mammalian - cytology
Embryonic Stem Cells - cytology
Humans
Mice
Mice, Inbred ICR
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Summary:To report the derivation and characterization of a new human embryonic stem cell (hESC) line NJGLLhES1. From the inner cell mass of frozen-thawed human embryos and with ICR mouse embryonic fibroblasts as the feeder layer, we established a new human embryonic stem cell line, which was named NJGLLhES1. We detected the karyotype of the cell line, determined the expressions of alkaline phosphatase, the specific cell surface antigens SSEA-3, SSEA-4, TRA-1-60, TRA-1-81 and the marker gene Oct-4, and examined the formation of embryoids and teratomas. NJGLLhES1 was maintained for over 1 year in vitro, with the morphological characteristics of hESC, a normal karyotype, positive expressions of alkaline phosphatase and specific cell marker genes, and the potential of forming embryoids and teratomas. A new human embryonic stem cell line NJGLLhES1 was successfully established, which remains karyotypically and phenotypically stable, undifferentiated and capable of self-renewal and pluripotential differentiation.
ISSN:1009-3591