Immunoproteomic assay of secretive proteins from Streptococcus suis type 2 strain SC84

To identify antigenic proteins secreted by Streptococcus suis (S. suis) type 2 strain SC84. Two-dimensional electrophoresis (2-DE), western-blot assay and matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) analysis were performed to search and identify antige...

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Bibliographic Details
Published inZhōnghuá liúxíngbìng zázhì Vol. 29; no. 3; p. 267
Main Authors Sun, Qiang-Zheng, Luo, Xia, Ye, Chang-Yun, Xiao, Di, Zheng, Han, Jing, Huai-Qi, Xu, Jian-Guo
Format Journal Article
LanguageChinese
Published China 01.03.2008
Subjects
Bacterial Proteins - analysis
Bacterial Proteins - immunology
Humans
Proteomics
Streptococcal Infections
Streptococcus suis - immunology
Streptococcus suis - isolation & purification
Streptococcus suis - metabolism
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Summary:To identify antigenic proteins secreted by Streptococcus suis (S. suis) type 2 strain SC84. Two-dimensional electrophoresis (2-DE), western-blot assay and matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) analysis were performed to search and identify antigenic proteins secreted by S. suis strain SC84, which triggered an outbreak of the disease in Sichuan province,China, in 2005. A total number of 14 western blot spots were found on PVDF membrane. 11 spots which could be found the existence of matching protein on coomassie G-250-stained 2-DE gel were identified by MALDI-TOF MS. The 11 proteins, all located at extra-cellular or cell wall, were classified into 8 kinds of proteins. Among of them, muramidase-released protein (MRP), suilysin (Sly) and extra-cellular factor (EF) were the known antigenic proteins, but several proteins such as putative 5'-nucleotidase, ribo-nucleases G and E, and predicted metal-loendo-peptidase were newly found antigenic proteins. All the id
ISSN:0254-6450