Experimental study on the effect of hypoxia on the expression of VEGF and TGF-beta1 of rat mandibular osteoblasts

To observe the effects of cobalt chloride (CoCl2)-simulated hypoxia on VEGF and TGF-beta1 expression and to provide theoretical basis for deciphering the molecular mechanism of clinical distraction osteogenesis. The mandibular osteoblasts were obtained from newborn Wistar rats within 24 hours and cu...

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Published inZhongguo xiu fu chong jian wai ke za zhi Vol. 22; no. 8; p. 984
Main Authors Guan, Jian, Wang, Jianping, Che, Xiaofei, Li, Dechao
Format Journal Article
LanguageChinese
Published China 01.08.2008
Subjects
Animals
Hypoxia - metabolism
Mandible - cytology
Osteoblasts - cytology
Osteoblasts - metabolism
Rats
Rats, Wistar
Transforming Growth Factor beta1 - biosynthesis
Vascular Endothelial Growth Factor A - biosynthesis
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Summary:To observe the effects of cobalt chloride (CoCl2)-simulated hypoxia on VEGF and TGF-beta1 expression and to provide theoretical basis for deciphering the molecular mechanism of clinical distraction osteogenesis. The mandibular osteoblasts were obtained from newborn Wistar rats within 24 hours and cultured and purified through modified enzymatic digestion. The morphological and histological changes of cells were evaluated by the HE staining, the histochemical staining for ALP, the collagen I immunohistochemistry staining and the calcified nodules staining, and the growth curves were drawn. The best cells of the 3rd-passage rats were treated with CoCl2, and then immunofluorescence was used to detect the expressions of VEGF and TGF-beta1 at 0, 3, 6, 9, 12 and 24 hours after culture. The HE staining demonstrated that the cellular forms were diverse, triangular, polygonal, circular and scaly and so on. The prominence varied in length and extended outwards. The nucleus was clearly discernible. The cytoplasma was ri
ISSN:1002-1892